作者: Jörg-Hermann Ozegowski , Heide Knöll , Dieter Gerlach , Werner Köhler
DOI: 10.1016/S0174-3031(84)80041-4
关键词:
摘要: Summary Erythrogenic toxin (ET) type C was purified from culture filtrates of Streptococcus pyogenes strains NY 5, T 18, and AT 13. Methods used included ammonium sulfate precipitation, phosphate column chromatography on CM-Sepharose Sephadex G 100, isoelectric focusing in gel. The molecular weight determined by SDS gel electrophoresis as 25,500. preparation reacted only with homologous antiserum (anti-C), but not antitoxins types A or B double diffusion tests. point to be 6.8 analytical focusing. Also the amino acid composition determined. found mitogenic (as well pyrogenic toxic for rabbits). ET is digested trypsin, pepsin, chymotrypsin Pronase E, rather stable when treated papain streptococcal proteinase.