Tumor-suppression function of transcription factor USF2 in prostate carcinogenesis

摘要: Although the transcription factor USF2 has been implicated in regulation of cellular growth and proliferation, it is unknown whether alterations contribute to tumorigenesis tumor development. We examined role prostate tumorigenesis. Western blot analysis revealed markedly decreased levels three androgen-independent cancer cell lines, PC-3, DU145, M12, as compared nontumorigenic epithelial cells or androgen-dependent line, LNCaP. Ectopic expression PC-3 did not affect proliferation rate on plastic surfaces. However, dramatically anchorage-independent soft agar (90–98% inhibition) invasion capability (80% matrix gel assay. Importantly, inhibited tumorigenicity an vivo nude mice xenograft model (80–90% inhibition). These results suggest that tumor-suppression function. Consistent with its function suppression, we found protein present normal but absent 18 42 (43%) human tissues (P=0.015). To further examine functional vivo, generated genetic deletion gene. USF2-null displayed marked hyperplasia at a young age, suggesting involved differentiation prostate. Together, these studies demonstrate tumor-suppressor plays carcinogenesis.