Chronic Delivery of Antibody Fragments Using Immunoisolated Cell Implants as a Passive Vaccination Tool
作者： Osiris Marroquin Belaunzaran , Maria Isabel Cordero , Veronica Setola , Siro Bianchi , Carmela Galli
DOI: 10.1371/JOURNAL.PONE.0018268
关键词:
摘要: BACKGROUND: Monoclonal antibodies and antibody fragments are powerful biotherapeutics for various debilitating diseases. However, high production costs, functional limitations such as inadequate pharmacokinetics tissue accessibility the current principal disadvantages broadening their use in clinic. METHODOLOGY AND PRINCIPAL FINDINGS: We report a novel method long-term delivery of fragments. designed an allogenous immunoisolated implant consisting polymer encapsulated myoblasts engineered to chronically release scFv targeted against N-terminus Aβ peptide. Following 6-month intracerebral therapy we observed significant reduction aggregation peptide APP23 transgenic mouse model Alzheimer's disease. In addition, assessment showed prevention behavioral deficits related anxiety memory traits. CONCLUSIONS SIGNIFICANCE: The chronic local using cell implants represents alternative passive vaccination strategy This technique could potentially benefit other diseases presently treated by systemic administration.
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