Lipase from Solvent-Tolerant Pseudomonas sp. DMVR46 Strain Adsorb on Multiwalled Carbon Nanotubes: Application for Enzymatic Biotransformation in Organic Solvents.
作者： Patel Vrutika , Madamwar Datta
DOI: 10.1007/S12010-015-1816-7
关键词:
摘要: Immobilization of biocatalysts onto particulate carriers has been widely explored for recycling biocatalyst. However, surface properties often affect the amount immobilized, their bioactivity and stability, hampering wide applications. The aim this work was to elucidate importance nanoimmobilization system in organic synthesis. multiwalled carbon nanotubes (MWCNTs) functionalized with a mixture concentrated acids create an interface enzyme immobilization. Successful functionalization immobilization structurally evidenced by transmision electron microscopy analysis Fourier-transform infrared spectroscopy analysis. Furthermore, immobilized exploited synthesis flavoured ester ethyl butyrate presence n-heptane. Optimized conditions enhanced found be 8.5 pH, 40 °C, 150 rpm, 0.15:0.2 M substrate molar ratio (ethanol/butyric acid) n-heptane as reaction medium. Utmost 81% obtained using lipase quite higher comparison that free lipase. activation energy indicated lower requirement on MWCNTs. In summary, MWCNTs simple adsorption method displayed excellent stability reusability, indicating its potential application
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