Lysis of intraventricular blood clot with urokinase in a canine model: Part 2. In vivo safety study of intraventricular urokinase.

摘要: It was determined from in vitro experiments that the minimal dose of urokinase required to lyse 10 ml clotted canine blood within a closed space must exceed 10,000 IU. We empirically doubled this minimum effective and tested vivo safety injecting 20,000 IU every 12 hours for 4 days into ventricles six adult mongrel dogs through an implanted catheter-reservoir system. The animals were monitored carefully local systemic bleeding by neurological clinical examination, hematological tests reflecting fibrinolytic status, serial computed tomography, postmortem histological examinations brain, meninges, peripheral organs. found intraventricular regimen did not cause intracranial hemorrhage even though had recent brain wounds related transcerebral ventricular catheterization. Mild activation fibrinolysis, implying passage enzyme ventricle blood, occurred 6 after each injection, but no hemorrhages seen. This also acute or chronic inflammatory changes meninges disturb cerebrospinal fluid circulation.