DETECTION OF THE TOMATO MI 1.2 GENE BY PCR USING NON-ORGANIC DNA PURIFICATION
作者： I. F. Bendezu
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摘要: A PCR based method using DNA extracted with non-organic reagents was developed to detect the presence of two alleles (Mi and mi) tomato Mi 1.2 resistance gene in plant material. Plant tissue from young leaves plants blotted onto a FTA® Card (Whatman, Inc.) processed according manufacturer's instructions. Individual samples containing template were used reactions. The forward primer (IMOF1) anneals 3' end conserved region beginning intron, reverse (IMOR1) C terminal leucine-rich repeat (LRR), amplifying 998 bp fragment corresponding marker for allele (Mi) 900 susceptible (mi). Tomato cultivars Motelle Motaci (isogenic locus), L. peruvianium f. glandulosumcv. Canta, esculentum var. cerasiforme VFNT Cherry, Mossol, Motabo, G-178 VFN-8 showed homozygous resistant genotype (Mi/Mi). Alicante, Harzfeuer F1, Marglobe, Marmande Rutgers (mi/mi) cultivar Big Boy both markers, heterozygous (Mi/mi). chi-square value these markers [chio= 1.2. Two advanced lines P1 (Mi/Mi) P2 13 individual their hybrid F[sub1] progeny also analyzed, mimarkers present (P1) parent (P2), respectively, all individuals. This combination results fast, accurate, cost effective reliable use marker-assisted selection breeding programmes model studies host root-knot nematode interaction.
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