The detection of smooth muscle desmin-like protein in BHK21/C13 fibroblasts.

摘要: Three distinct proteins, actin (42,000 daltons), the principal form of fibroblast 10 nm filament protein (55,000 and a with molecular weight 52,000 pI 5.8 were detected in nonionic detergent-insoluble cytoskeletal preparations control BHK21/C13 line 9 hamster fibroblasts. Cytoskeletal other cell types, such as NIL8 primary embryo fibroblasts, contained 55,000-dalton component but lacked 52,000-dalton protein. A Rous sarcoma virus transformant an adenovirus 9, resembled types that they only 55,000- 42,000-dalton polypeptides. The identity cells was studied. This co-electrophoreses on both one- two-dimensional polyacrylamide gels predominant muscle Further, one-dimensional peptide maps smooth are identical to one another from 42,000- components preparations. We conclude contain