Burkholderia genome analysis reveals new enzymes belonging to the nitrilase superfamily: The amidase of Burkholderia cepacia (hospital isolate)
作者： C. Novo , R. Tata , A. Clemente , P.R. Brown
DOI: 10.1016/J.IJBIOMAC.2003.08.002
关键词:
摘要: Burkholderia cepacia (formerly Pseudomonas cepacia) grows in media containing acetamide or propionamide as C and N sources. Chromosomal DNA from a hospital isolate of B. served template PCRs using primers designed for the amplification P. aeruginosa amiE gene that encodes an aliphatic amidase. Partial sequencing PCR products gave translated sequence 100% identical with amino acid A search genomes detected putative amidase J2315 high identity to predicted other species also possessed CN_hydrolases use same catalytic triad (Glu-Lys-Cys) Superimposition theoretical three-dimensional models suggested differences sequences between amidases (hospital isolate) do not affect their structure.
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