Quarterly Report, July - September 1989

摘要: Abstract : The first quarter has been devoted in part to organization, ordering, and reagent preparation. We have ordered, obtained, are the process of setting up computer work station, beta scintillation counter low pressure chromatography system. As plan we made killed whole cell vaccines E. coli 0111B4 S. typhimurium, generated polyclonal rabbit antisera each that titers 1:3000,000 by ELISA. now purifying IgG on DEAE sephacel anion exchange columns, this week coupling typhimurium LPS sepharose beads order affinity- purify IgG. If procedure goes without difficulty, will couple next several weeks also. ordered rabbits for preparation large pools normal tolerant sera use as constant beginning material purification. also started set cytotoxicity assay tumor necrosis factor using 929 cells, a task easier modern culture facility new laboratory. Our early results suggest murine macrophage line (RAW 264.7) serve source (alleviating cumbersome need peritoneal cells), our preliminary with these cells indicate is sensitive picograms/ml LPS. try assay, addition limulus lysate, an check neutralizing ability preparations during