A new non-canonical pathway of Gαq protein regulating mitochondrial dynamics and bioenergetics
作者： Cristiane Benincá , Jesús Planagumà , Adriana de Freitas Shuck , Rebeca Acín-Perez , Juan Pablo Muñoz
DOI: 10.1016/J.CELLSIG.2014.01.009
关键词:
摘要: Contrary to previous assumptions, G proteins do not permanently reside on the plasma membrane, but are constantly monitoring cytoplasmic surfaces of membrane and endomembranes. Here, we report that Gαq Gα11 locate at mitochondria play a role in complex signaling pathway regulates mitochondrial dynamics. Our results provide evidence for presence heteromeric protein (Gαq/11βγ) outer inner membrane. Both localizations necessary maintain proper equilibrium between fusion fission; which is achieved by altering activity mitofusin proteins, Drp1, OPA1 potential both membranes. As result absence Gαq/11, there decrease rates overall respiratory capacity, ATP production OXPHOS-dependent growth. These findings demonstrate serves as physiological function: stabilizing elongated regulating energy Drp1 Opa1 dependent mechanisms. This thereby links organelle dynamics physiology.
sci-hub.se 参考文章(80)
Miriam Masià-Balagué, Ismael Izquierdo, Georgina Garrido, Arnau Cordomí, Laura Pérez-Benito, Nichol L. G. Miller, David D. Schlaepfer, Véronique Gigoux, Anna M. Aragay, Gastrin-stimulated Gα13Activation of Rgnef Protein (ArhGEF28) in DLD-1 Colon Carcinoma Cells Journal of Biological Chemistry. ,vol. 290, pp. 15197- 15209 ,(2015) , 10.1074/JBC.M114.628164
A. Leyte, F.A. Barr, R.H. Kehlenbach, W.B. Huttner, Multiple trimeric G-proteins on the trans-Golgi network exert stimulatory and inhibitory effects on secretory vesicle formation. The EMBO Journal. ,vol. 11, pp. 4795- 4804 ,(1992) , 10.1002/J.1460-2075.1992.TB05585.X
Hermann Schägger, Native electrophoresis for isolation of mitochondrial oxidative phosphorylation protein complexes. Methods in Enzymology. ,vol. 260, pp. 190- 202 ,(1995) , 10.1016/0076-6879(95)60137-6
Kendra Bence, Wei Ma, Tohru Kozasa, Xin-Yun Huang, Direct stimulation of Bruton's tyrosine kinase by G q -protein α-subunit Nature. ,vol. 389, pp. 296- 299 ,(1997) , 10.1038/38520
J. R. Hepler, D. M. Berman, A. G. Gilman, T. Kozasa, RGS4 and GAIP are GTPase-activating proteins for Gqα and block activation of phospholipase Cβ by γ-thio-GTP-Gqα Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 428- 432 ,(1997) , 10.1073/PNAS.94.2.428
Stefan Offermanns, Li‐Ping Zhao, Antje Gohla, Ildiko Sarosi, Melvin I Simon, Thomas M Wilkie, Embryonic cardiomyocyte hypoplasia and craniofacial defects in Gαq. Gα11-mutant mice The EMBO Journal. ,vol. 17, pp. 4304- 4312 ,(1998) , 10.1093/EMBOJ/17.15.4304
R.G. Anderson, P. Liu, B.K. Pilcher, W.P. Li, Cell-specific targeting of caveolin-1 to caveolae, secretory vesicles, cytoplasm or mitochondria. Journal of Cell Science. ,vol. 114, pp. 1397- 1408 ,(2001) , 10.1242/JCS.114.7.1397
Yo Sugawara, Hiroko Nishii, Tomoko Takahashi, Junji Yamauchi, Norikazu Mizuno, Kenji Tago, Hiroshi Itoh, The lipid raft proteins flotillins/reggies interact with Gαq and are involved in Gq-mediated p38 mitogen-activated protein kinase activation through tyrosine kinase Cellular Signalling. ,vol. 19, pp. 1301- 1308 ,(2007) , 10.1016/J.CELLSIG.2007.01.012
Sanjay W. Pimplikar, Kai Simons, Regulation of apical transport in epithelial cells by a Gs class of heterotrimeric G protein Nature. ,vol. 362, pp. 456- 458 ,(1993) , 10.1038/362456A0
M. D. Resh, Palmitoylation of Ligands, Receptors, and Intracellular Signaling Molecules Science Signaling. ,vol. 2006, ,(2006) , 10.1126/STKE.3592006RE14