Thrombin-linked aptamer assay for detection of platelet derived growth factor BB on magnetic beads in a sandwich format
作者： Limin Guo , Qiang Zhao
DOI: 10.1016/J.TALANTA.2016.05.037
关键词:
摘要: Here we describe a thrombin-linked aptamer assay (TLAA) for protein by using thrombin as an enzyme label, harnessing activity of and affinity binding. TLAA converts detection specific target proteins to the DNA sequence that consists two aptamers with first binding second thrombin. Through binding, is labeled on target, catalyzes hydrolysis small peptide substrate into product, generating signals quantification. As proof principle, show sandwich platelet derived growth factor BB (PDGF-BB) anti-PDGF-BB antibody coated magnetic beads oligonucleotide containing PDGF-BB The both results in labeling complex We achieved at 16 pM. This contributes new application its bioanalysis, shows potentials developments.
core.ac.uk
sci-hub.se 参考文章(57)
Richard Lottenberg, Ulla Christensen, Craig M. Jackson, Patrick L. Coleman, Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates. Methods in Enzymology. ,vol. 80, pp. 341- 361 ,(1981) , 10.1016/S0076-6879(81)80030-4
Xi Wang, Wei Li, Zhonghui Li, Hui Li, Danke Xu, A highly sensitive fluorescence turn-on platform with silver nanoparticles aptasening for human platelet-derived growth factor-BB. Talanta. ,vol. 144, pp. 1273- 1278 ,(2015) , 10.1016/J.TALANTA.2015.07.019
Yeon Seok Kim, Nurul Hanun Ahmad Raston, Man Bock Gu, Aptamer-based nanobiosensors Biosensors and Bioelectronics. ,vol. 76, pp. 2- 19 ,(2016) , 10.1016/J.BIOS.2015.06.040
C.H. Heldin, Structural and functional studies on platelet‐derived growth factor. The EMBO Journal. ,vol. 11, pp. 4251- 4259 ,(1992) , 10.1002/J.1460-2075.1992.TB05523.X
Q Wu, M Tsiang, J.E. Sadler, Localization of the single-stranded DNA binding site in the thrombin anion-binding exosite. Journal of Biological Chemistry. ,vol. 267, pp. 24408- 24412 ,(1992) , 10.1016/S0021-9258(18)35781-8
Meral Yüce, Naimat Ullah, Hikmet Budak, None, Trends in aptamer selection methods and applications Analyst. ,vol. 140, pp. 5379- 5399 ,(2015) , 10.1039/C5AN00954E
Louis C. Bock, Linda C. Griffin, John A. Latham, Eric H. Vermaas, John J. Toole, Selection of single-stranded DNA molecules that bind and inhibit human thrombin. Nature. ,vol. 355, pp. 564- 566 ,(1992) , 10.1038/355564A0
Supriya Pai, Ana Roberts, Andrew D Ellington, Aptamer amplification: divide and signal Expert Opinion on Medical Diagnostics. ,vol. 2, pp. 1333- 1346 ,(2008) , 10.1517/17530050802562016
Saw Yi Toh, Marimuthu Citartan, Subash C.B. Gopinath, Thean-Hock Tang, Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay. Biosensors and Bioelectronics. ,vol. 64, pp. 392- 403 ,(2015) , 10.1016/J.BIOS.2014.09.026
Juewen Liu, Zehui Cao, Yi Lu, Functional Nucleic Acid Sensors Chemical Reviews. ,vol. 109, pp. 1948- 1998 ,(2009) , 10.1021/CR030183I