MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells
作者： YONG GUO , YANG WANG , YINQIN LIU , YONGMING LIU , QIANGCHENG ZENG
关键词:
摘要: MicroRNA (miRNA) is an important regulator of cell differentiation and function. Mechanical strain in the growth osteoblasts. Therefore, mechanresponsive miRNA may be response osteoblasts to mechanical strain. The purpose present study was select identify mechanoresponsive miRNAs Mouse osteoblastic MC3T3-E1 cells were cultured culture dishes stimulated with a tensile 2,50 μe at 0.5 Hz, activity alkaline phosphatase (ALP), mRNA levels ALP, osteocalcin (OCN), collagen type I (Col I), protein bone morphogenetic proteins (BMPs) medium assayed. Following microarray reverse transcription-quantitative polymerase chain reaction analyses, differentially expressed mechanically strained unstrained selected identified. Using bioinformatics analysis, target genes then predicted. results revealed that 2,500 increased OCN Col I, protein(BMP)-2 BMP-4 Continuous stimulation for 8 h had most marked stimulant effects. miR-218, miR-191*, miR-3070a miR-33 identified as cells. Certain these four involved differentiation. These findings indicated μe, particularly period h, promoted differentiation, potential their
pubmed.cn参考文章(37)
David P Bartel, MicroRNAs: Genomics, Biogenesis, Mechanism, and Function Cell. ,vol. 116, pp. 281- 297 ,(2004) , 10.1016/S0092-8674(04)00045-5
Vimalraj S, Selvamurugan N, MicroRNAs: Synthesis, Gene Regulation and Osteoblast Differentiation. Current Issues in Molecular Biology. ,vol. 15, pp. 7- 18 ,(2013)
Yukiko Nakano, Jennifer Forsprecher, Mari T. Kaartinen, Regulation of ATPase activity of transglutaminase 2 by MT1-MMP: implications for mineralization of MC3T3-E1 osteoblast cultures. Journal of Cellular Physiology. ,vol. 223, pp. 260- 269 ,(2009) , 10.1002/JCP.22034
T. Kaneuji, S. Nogami, W. Ariyoshi, T. Nishihara, T. Takahashi, Regulatory effect on osteoclastogenesis of mechanical strain-loaded osteoblasts International Journal of Oral and Maxillofacial Surgery. ,vol. 40, pp. 1215- ,(2011) , 10.1016/J.IJOM.2011.07.640
Charles H. Turner, Fredrick M. Pavalko, Mechanotransduction and functional response of the skeleton to physical stress: the mechanisms and mechanics of bone adaptation Journal of Orthopaedic Science. ,vol. 3, pp. 346- 355 ,(1998) , 10.1007/S007760050064
Tzu-Hung Lin, Rong-Sen Yang, Chih-Hsin Tang, Ming-Yueh Wu, Wen-Mei Fu, Regulation of the maturation of osteoblasts and osteoclastogenesis by glutamate. European Journal of Pharmacology. ,vol. 589, pp. 37- 44 ,(2008) , 10.1016/J.EJPHAR.2008.04.060
Patricia Ducy, Rui Zhang, Valérie Geoffroy, Amy L Ridall, Gérard Karsenty, Osf2/Cbfa1: A Transcriptional Activator of Osteoblast Differentiation Cell. ,vol. 89, pp. 747- 754 ,(1997) , 10.1016/S0092-8674(00)80257-3
L.E. Lanyon, C.T. Rubin, Static vs dynamic loads as an influence on bone remodelling Journal of Biomechanics. ,vol. 17, pp. 897- 905 ,(1984) , 10.1016/0021-9290(84)90003-4
Anna M. Szczesniak, Robert W. Gilbert, Maya Mukhida, Gail I. Anderson, Mechanical loading modulates glutamate receptor subunit expression in bone. Bone. ,vol. 37, pp. 63- 73 ,(2005) , 10.1016/J.BONE.2003.10.016
Yuji Ito, Daisuke Inoue, Shinsuke Kido, Toshio Matsumoto, c-Fos degradation by the ubiquitin–proteasome proteolytic pathway in osteoclast progenitors Bone. ,vol. 37, pp. 842- 849 ,(2005) , 10.1016/J.BONE.2005.04.030