Slotoxin, αKTx1.11, a new scorpion peptide blocker of MaxiK channels that differentiates between α and α+β (β1 or β4) complexes
作者： Jesus Garcia-Valdes , Fernando Z Zamudio , Ligia Toro , Lourival D Possan
DOI: 10.1016/S0014-5793(01)02791-0
关键词:
摘要: A novel peptide from Centruroides noxius Hoffmann scorpion venom was isolated and sequenced. The 37 amino acid belongs to the charybdotoxin sub-family (alphaKTx1) numbered member 11. alphaKTx1.11 has 75% sequence identity with iberiotoxin 54% charybdotoxin. revealed specificity for mammalian MaxiK channels (hSlo), thus, named slotoxin. Slotoxin blocks pore-forming alpha subunit reversibly (K(d)=1.5 nM). association alpha+beta (beta1 or beta4) approximately 10 times slower than charybdotoxin, leading a lack of effect on alpha+beta4 when tested at 100 nM 5 min. Thus, slotoxin is better tool distinguish complexes.
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