ATP-Binding Cassette Properties of Recombination Mediator Protein RecF

摘要: 1.1 Recombinational repair Homologous recombination (HR) is essential for genetic diversity and genome stability. The conserved RecA-like recombinases promote pairing consequent exchange of fragments between two homologous DNA molecules during conjugation in bacteria meiotic eukaryotes. HR a main pathway particularly important case large-scale damages, including chromosome or double-stranded (ds) breaks (DSBs) long single-stranded (ss) gaps (SSGs) (Cox, 1991; Kowalczykowski et al., 1994). broken chain paired with the intact DNA, which serves as template synthesis damaged DNA. same are also involved origin-independent restart stalled replication, frequently occurring event every cell (Cox 2000; Kowalczykowski, Kuzminov, 2001). initiated by cooperative binding RecA recombinase to ssDNA hundreds thousands nucleotides forming nucleoprotein filament, so called presynaptic complex often designated RecA*. can bind dsDNA strands. RecA* has multiple activities beyond strand invasion (Figure 1). Those include triggering damage SOS response through stimulation LexA autocleavage (Rehrauer 1996) activation UmuD subunit error-prone polymerase PolV translesion bypass small-scale errors (Jiang 2009; Rajagopalan 1992). was suggested stabilize maintain replication fork (Courcelle 1997). Consequently, regulated at levels 2007).