Production and characterization of cellulolytic and xylanolytic enzymes from the ligninolytic white-rot fungus Phanerochaete chrysosporium grown on sugarcane bagasse
作者： A.I. Khalil
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摘要: The ligninolytic white-rot fungus Phanerochaete chrysosporium BKM-F-1767 produced extracellular cellulolytic enzymes (carboxymethylcellulase, CMCase and β-glucosidase) xylanolytic (xylanase β-xylosidase) in liquid medium containing 1.0% sugarcane bagasse with or without glucose. changes pH soluble protein content were monitored the culture filtrates. results obtained showed that decreased after 3 days then increased. increased reached maximum value 12 days. activities of higher case characterization study indicated optimum values 4.6, 4.2, 5.0 for CMCase, β-glucosidase, xylanase β-xylosidase, respectively temperatures 60, 70, 65 60 °C investigated enzymes, respectively. also prolonged heating (5 h) at °C, β-xylosidase retained 81.2, 86.8, 51.5 27.4% activity,
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