Induction of proliferation of purified human myeloid progenitor cells: a rapid assay for granulocyte colony-stimulating factors.

摘要: The proliferation and differentiation of granulocyte monocyte progenitor cells (CFU-C) in vitro is dependent on the presence a group closely related glycoproteins termed colony-stimulating factors (CSF). In order to investigate interaction these with CFU-C, we purified CFU-C from peripheral blood chronic myeloid leukemia patients an immune rosette technique using specific monoclonal antibodies (mean 74-fold enrichment, 45% cloning efficiency). Colony formation by demonstrated absolute dependence exogenous source CSF. Liquid culture small aliquots enriched CSF-containing medium resulted rapid, time- concentration-dependent induction DNA synthesis as measured 3H-thymidine incorporation. This CSF was used develop microassay system for activity. activity could be reproducibly quantitated 24–48 hr. proliferating this assay were shown examining morphology their progeny determining surface antigen phenotype responding (Ia+, T3-, B1-, Mo1-). provides quantitative assessment that may useful purification human generation structures.