Single-Molecule Imaging of Individual Amyloid Protein Aggregates in Human Biofluids

摘要: The misfolding and aggregation of proteins into amyloid fibrils characterizes many neurodegenerative disorders such as Parkinson’s Alzheimer’s diseases. We report here a method, termed SAVE (single aggregate visualization by enhancement) imaging, for the ultrasensitive detection individual oligomers using single-molecule fluorescence microscopy. demonstrate that this method is able to detect presence aggregates α-synuclein, tau, amyloid-β. In addition, we show can also be identified in human cerebrospinal fluid (CSF). Significantly, see twofold increase average concentration CSF from disease patients compared age-matched controls. Taken together, conclude provides an opportunity characterize structural nature key biofluid, therefore has potential study progression both animal models humans enhance our understanding of...