Transcriptomic analysis suggests the inhibition of DNA damage repair in green alga Raphidocelis subcapitata exposed to roxithromycin.

摘要: Abstract Macrolide antibiotics are common contaminants in the aquatic environment. They toxic to a wide range of primary producers, inhibiting algal growth and further hindering delivery several ecosystem services. Yet molecular mechanisms macrolides algae remain undetermined. The objectives this study were therefore to: 1. evaluate whether at environmentally relevant level inhibit algae; 2. test hypothesis that bind ribosome protein translocation algae, as it does bacteria. In study, transcriptomic analysis was applied elucidate toxicological mechanism model green alga Raphidocelis subcapitata treated with 5 90 μg L−1 typical macrolide roxithromycin (ROX). While exposure ROX 5 μg L−1 for 7 days did not affect transciptome, resulted 45% inhibition 2306 (983 up- 1323 down-regulated) DEGs, which primarily enriched metabolism energy, lipid, vitamins, DNA replication repair pathways. Nevertheless, genes involved pathways relation translation processing dysregulated. Surprisingly, we found base excision process mostly repressed, suggesting may be genotoxic cause damage R. subcapitata. Taken together, unlikely pose threat environment mode action bacteria directly extrapolated algae.