Comparison between different bio-treatments of a hydrocarbon contaminated soil from a landfill site

摘要: We investigated the bio-remediation of a hydrocarbon contaminated soil pile that was slated for landfill disposal, by utilising laboratory based-soil microcosms. The objective to accelerate reduction total petroleum (TPH) levels could potentially allow be used outside site. Soil TPH content reduced 57% over 2 year period from 15,800 ~6,800 mg kg -1 in untreated pile, making eligible disposal under current Australian legislation. Subsequent (natural attenuation, biostimulation, bioaugmentation and biostimulation-bioaugmentation) resulted 74% (~1,800 ) 56 days with most occurring first 21 (~60%). Nutrient microbial amendments did not confer any long-term benefit on rate natural attenuation being equally efficient as other bioremediation strategies at day 56. bioavailability assay showed linear decrease an initial 84 35% explain rates observed after 21. Denaturing gradient gel electrophoresis (DGGE) analyses 16S rDNA internal transcribed spacer regions genes revealed diverse stable (unaffected amendments) bacterial fungal communities. Microbial analysis also substantial populations alkane (alkB) degrading bacteria soils. This study therefore soils possess capacity, which can exploited greater removal through attenuation. lower than 1,800 obtained this provided contaminant is made more bio-available. would use such productive purposes sites extending lifespan these sites. Key words: Bioremediation, denaturing (DGGE), rDNA, ITS, alkB