Development of a qualitative indirect ELISA for the measurement of rabies virus-specific antibodies from vaccinated dogs and cats

摘要: A protocol suitable for the detection of rabies virus-specific antibodies in serum samples from companion animals using an enzyme linked immunosorbent assay (ELISA) is described. This method has been used successfully qualitative assessment a cohort vaccinated dogs and cats. In two initial field studies, variable population Veterinary Laboratories Agency (VLA), United Kingdom were tested. first study (n = 1000), number false-positive false-negative results was 11 (1.1%) 67 (6.7%), respectively. second 920), 7 (0.8%) 52 (5.7%). third study, undertaken at l'Agence Francaise de Securite Sanitaire des Aliments (AFSSA), Nancy, France 440), 1 sample (0.23%) 91 (20.7%) identified. Data generated this prototype ELISA indicate strong correlation specificity when compared to gold standard fluorescent antibody virus neutralisation (FAVN) test. Although lower sensitivity than FAVN test, it useful tool rapidly screening animals. Using cut-off value 0.6 EU/ml, (R % VLA 79% AFSSA) 97.3%) indices between favourably with data The major advantages test are that can be completed four hours, does not require use live performed without need specialised laboratory containment. contrasts 4 days conventional assays. current format, described would valuable domestic combination other Office International Epizooties (OIE) accepted serological tests.