The interplay at the replisome mitigates the impact of oxidative damage on the genetic integrity of hyperthermophilic Archaea.
作者： Tom Killelea , Adeline Palud , Farida Akcha , Mélanie Lemor , Stephane L'haridon
DOI: 10.7554/ELIFE.45320
关键词:
摘要: 8-oxodeoxyguanosine (8-oxodG), a major oxidised base modification, has been investigated to study its impact on DNA replication in hyperthermophilic Archaea. Here we show that 8-oxodG is formed the genome of growing cells, with elevated levels following exposure oxidative stress. Functional characterisation cell-free extracts and polymerisation enzymes, PolB, PolD, p41/p46 complex, alone or presence accessory factors (PCNA RPA) indicates translesion synthesis occurs under replicative conditions. One effects was stalling, but each individual proteins could insert extend past differing efficiencies. The introduction RPA PCNA influenced PolB PolD similar ways, yet provided cumulative enhancement performance p41/p46. Overall, seen be potentially mutagenic leading errors are reminiscent dA:8-oxodG pairing.
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