Nitric oxide synthesis in rat peritoneal macrophages is induced by IgE/DNP complexes and cyclic AMP analogues. Evidence in favor of a common signaling mechanism.

摘要: The production of nitric oxide was studied in rat adherent peritoneal cells stimulated with preformed IgE/DNP-BSA complexes made IgE obtained from a secreting hybridoma. Stimulation at equivalence induced both the NO and an increased expression mRNA inducible isoform synthase (iNOS). Production also elicited by rabbit polyclonal F(ab')2 anti-CD23 cross-reacting CD23. Because did not elicit Ca2+ mobilization genistein influence NO, cyclic AMP considered as alternative signaling molecule. Combination suboptimal concentration dibutyryl showed additive effect on production, whereas this observed when agonists were used supraoptimal doses. inhibitor AMP-specific phosphodiesterase IV, rolipram, which acts enzyme predominantly expressed inflammatory cells, NO. Furthermore, intracellular levels AMP. Taken together, these data indicate that stimulation mononuclear phagocytes via low-affinity receptor Fc epsilon RII or rising concentrations leads to enhanced iNOS. Evidence favor involvement pathway linked is provided produced complexes.