Bilirubin induces apoptosis and necrosis in human NT2-N neurons.
作者： Erik Hankø , Thor Willy Ruud Hansen , Runar Almaas , Julie Lindstad , Terje Rootwelt
DOI: 10.1203/01.PDR.0000148711.11519.A5
关键词:
摘要: Studies on primary cultures of newborn rodent neurons have suggested that neuronal death induced by unconjugated bilirubin (UCB) is mainly apoptotic in nature. We exposed a human teratocarcinoma-derived cell line, NT2-N neurons, to different concentrations UCB and albumin at 1.5 molar ratio used multiple, independent measures damage evaluate injury after 6, 24, 48 h. Low doses (0.66, 2, 5 microM) moderate loss 3-4[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) cleavage accompanied delayed morphologic changes consistent with apoptosis (2 microM). Moderate (10 25 resulted early (6 h) necrosis subset while remaining underwent progressive impairment MTT increasing lactate dehydrogenase (LDH) release predominantly apoptosis. High (100 severe cleavage, extensive LDH release, within 6 Used as positive control for apoptosis, 2 microM STS over the entire observation period. DNA electrophoresis h was compatible both treatment
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sci-hub.se 参考文章(31)
Stephen D. Zucker, Wolfram Goessling, Mechanism of hepatocellular uptake of albumin-bound bilirubin. Biochimica et Biophysica Acta. ,vol. 1463, pp. 197- 208 ,(2000) , 10.1016/S0005-2736(99)00196-0
Runar Almaas, Morten Pytte, Julie K. Lindstad, Marianne Wright, Ola Didrik Saugstad, David Pleasure, Terje Rootwelt, Acidosis has opposite effects on neuronal survival during hypoxia and reoxygenation. Journal of Neurochemistry. ,vol. 84, pp. 1018- 1027 ,(2003) , 10.1046/J.1471-4159.2003.01593.X
Torben R. Neelands, Anthony P. J. King, Robert L. Macdonald, Functional expression of L-, N-, P/Q-, and R-type calcium channels in the human NT2-N cell line. Journal of Neurophysiology. ,vol. 84, pp. 2933- 2944 ,(2000) , 10.1152/JN.2000.84.6.2933
E. Bonfoco, D. Krainc, M. Ankarcrona, P. Nicotera, S. A. Lipton, Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 7162- 7166 ,(1995) , 10.1073/PNAS.92.16.7162
Marcel Leist, Pierluigi Nicotera, None, Apoptosis, Excitotoxicity, and Neuropathology Experimental Cell Research. ,vol. 239, pp. 183- 201 ,(1998) , 10.1006/EXCR.1997.4026
Rui F.M. Silva, Cecı́lia M.P. Rodrigues, Dora Brites, Bilirubin-induced apoptosis in cultured rat neural cells is aggravated by chenodeoxycholic acid but prevented by ursodeoxycholic acid. Journal of Hepatology. ,vol. 34, pp. 402- 408 ,(2001) , 10.1016/S0168-8278(01)00015-0
Richard A. Weisiger, J. Donald Ostrow, Ronald K. Koehler, Cecile C. Webster, Pasupati Mukerjee, Lorella Pascolo, Claudio Tiribelli, Affinity of human serum albumin for bilirubin varies with albumin concentration and buffer composition: results of a novel ultrafiltration method. Journal of Biological Chemistry. ,vol. 276, pp. 29953- 29960 ,(2001) , 10.1074/JBC.M104628200
Forough Nowrouzian, Bill Hesselmar, Robert Saalman, Inga-Lisa Strannegård, Nils Åberg, Agnes E Wold, Ingegerd Adlerberth, Escherichia coli in infants' intestinal microflora: colonization rate, strain turnover, and virulence gene carriage. Pediatric Research. ,vol. 54, pp. 8- 14 ,(2003) , 10.1203/01.PDR.0000069843.20655.EE
Maria Ankarcrona, Jeannette M. Dypbukt, Emanuela Bonfoco, Boris Zhivotovsky, Sten Orrenius, Stuart A. Lipton, Pierluigi Nicotera, Glutamate-induced neuronal death: A succession of necrosis or apoptosis depending on mitochondrial function Neuron. ,vol. 15, pp. 961- 973 ,(1995) , 10.1016/0896-6273(95)90186-8
Terje Rootwelt, Michelle Dunn, Marc Yudkoff, Takayuki Itoh, Runar Almaas, David Pleasure, Hypoxic cell death in Human NT2-N neurons : Involvement of NMDA and non-NMDA glutamate receptors Journal of Neurochemistry. ,vol. 71, pp. 1544- 1553 ,(2002) , 10.1046/J.1471-4159.1998.71041544.X