MicroRNA regulation during Nibea albiflora immuno-resistant against Cryptocaryon irritans challenge in fish skin

摘要: Abstract Nibea albiflora (Yellow drum) was discovered to be highly resistant the infection of Cryptocaryon irritans a lethal ciliate protozoa parasite during disease outbreak. Investigation miRNAs which post-transcriptionally regulate genes will deeper our understanding regulation immune response against infection. In this study, we challenged N. with C. at median concentration (2050 theronts/g fish). Skins from infected and controlled fish were sampled 24 h 72 h for miRNA sequencing. total, identified 180 known 71 novel miRNAs, in 30 significantly affected by infection, 19 influenced time 16 interaction between Totally, 7396 predicted as targeted regulated differential expressed genes. The enriched C-type lectin receptor pathway, MAPK signaling pathway mTOR closely related antigen recognition, presentation downstream signal transduction immuno-activation. Overall, data suggest that most intensively regulating gene expression involved rather than directly involve immunological factor