Dynamic Culture and Selective Extraction of Target Microbial Cells in Self-Assembled Particle Membrane-Integrated Microfluidic Bioreactor Array

摘要: Various microfluidic devices have overcome many disadvantages common to conventional bioreactor systems by enabling active manipulation of cell-culture conditions, monitoring cellular responses in high-throughput mode, and extraction target cells a relatively rapid low-cost manner. However, existing still limitations, including the complexity their operation lack availability dynamic control chemical environment. Here, we present novel array device capable not only stable programing environments but also selective cells. This comprises 64 microchambers 16 × 4 format, each microchamber is integrated with robust nanoporous membrane on one side an H-shaped entrance other. The made self-assembled particles allowed continuous sequential delivery various nutrients containing gene inducers compartmentalized microbial cells, thereby cell culturing. Additionally, was used for local blocking employing UV-curable material, retrieval from while sequestering nontarget microchambers. Our results demonstrated that targeted rare could be isolated separated mixture repeating procedure. Therefore, anticipate this will widely screening/extraction off-chip postanalyses microorganisms.