Protein Vaccines Induce Uncommitted IL-2-Secreting Human and Mouse CD4 T Cells, Whereas Infections Induce More IFN-γ-Secreting Cells

摘要: Mouse and human CD4 T cells primed during an immune response may differentiate into effector phenotypes such as Th1 (secreting IFN-gamma) or Th2 IL-4) that mediate effective immunity against different classes of pathogen. However, can also remain uncommitted, secreting IL-2 chemokines, but not IFN-gamma IL-4. We now show by protein vaccines mostly secreted IL-2, IFN-gamma, whereas in the same individuals most initially infection with live pathogens IFN-gamma. further demonstrate many tetanus-specific IL-2+IFN-gamma- are uncommitted a single cell following vitro stimulation under appropriate polarizing conditions. In contrast, influenza-specific maintained Th1-like phenotype even Th2-polarizing Similarly, adoptively transferred OTII transgenic mouse mainly after priming OVA alum, were biased toward secretion when peptide presented pathogen Ag infection. Thus, subunit prime unique subset differentiated, lack some functional properties committed effectors induced This has implications for design more requiring strong responses.