Luminescence detecting apparatuses and methods

作者: Israel Metal , George Sabak , Jeff A. Levi , Yongdong Wang , John C. Voyta

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摘要: A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent are placed in a plurality of sample wells tray, the tray visible-light impervious chamber containing charge coupled device camera. The may be injected wells, with buffers reagents, by an injector. In chamber, light from pass through collimator, Fresnel field lens, filter, camera whereupon focused image created optics on charge-coupled (CCD) use combination collimator reduces crosstalk between below level attainable prior art. Preferred embodiments disclosed include central processing control all operations, multiple wavelength filter wheel, robot handling reagents. software integrated invention elements mechanical alignment, outlier shaving, edge detection masking, manipulation integration times to expand dynamic range, correction, dark subtraction interpolation drift multi-component analysis applications specifically tailored luminescence, uniformity correction.