Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
作者： Richard George Antonius Bernardus Sewalt , Theodorus Hendrikus Jacobus Kwaks , Arie Pieter Otte
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摘要: The invention is concerned with means and methods for providing a cell protein expression unit comprising nucleic acid said encoding binding site member of chromatin modification system rendering more accessible transcription (opener), wherein opener present in cell. Preferred openers comprise histone proteins, remodeling proteins trithorax group or equivalents. cells thus generated acids such are provided. Openers preferred the context STAR TRAP sequences.
google.com.br 参考文章(90)
J Bielicki, M Fuller, X H Guo, C P Morris, J J Hopewood, D S Anson, Expression, purification and characterization of recombinant human N-acetylgalactosamine-6-sulphatase. Biochemical Journal. ,vol. 311, pp. 333- 339 ,(1995) , 10.1042/BJ3110333
A. Dusty Miller, Mohammed A. Adam, William Reginald Alfred Osborne, Cap-independent multicistronic retroviral vectors ,(1993)
J. Eggermont, N.J. Proudfoot, Poly(A) signals and transcriptional pause sites combine to prevent interference between RNA polymerase II promoters. The EMBO Journal. ,vol. 12, pp. 2539- 2548 ,(1993) , 10.1002/J.1460-2075.1993.TB05909.X
Kevin D. Wells, Juli A. Foster, Karen Moore, Vernon G. Pursel, Robert J. Wall, Codon optimization, genetic insulation, and an rtTA reporter improve performance of the tetracycline switch. Transgenic Research. ,vol. 8, pp. 371- 382 ,(1999) , 10.1023/A:1008952302539
Edward M. Rockenstein, Michael A. Whitney, Non-optimal Kozaks sequences ,(1998)
Stefan Kochanek, Gudrun Schiedner, Permanent amniocytic cell line, its production and use for the production of gene transfer vectors ,(2000)
Christopher Robert Bebbington, Trish Benton, Shao Xiang, Robert Crombie, Karla Ann Henning, David J. King, Compositions and methods for high-level, large-scale production of recombinant proteins ,(2002)
Arthur David Levinson, Christian Clinton Simonsen, Polycistronic expression vector construction ,(1983)
Chikh Bengra, Dorothy Tuan, Qiaoming Long, Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors ,(1999)
William F. Thompson, Steven Spiker, George C. Allen, Gerald Hall, Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells ,(1996)