Chlamydial Serology: Comparative Diagnostic Value of Immunoblotting, Microimmunofluorescence Test, and Immunoassays Using Different Recombinant Proteins as Antigens

摘要: To improve the reliability of serodiagnosis Chlamydia trachomatis infections, an immunoblot analysis, a microimmunofluorescence titration, and different immunoassays using synthetic peptides derived from species-specific epitopes in variable domain IV major outer membrane protein or recombinant antigens (heat shock 70 [hsp70], hsp60, hsp10, polypeptide encoded by open reading frame 3 plasmid [pgp3], macrophage infectivity potentiator, fragment total lipopolysaccharide) were evaluated. Because cross-reactions between chlamydial species have been reported, tests also performed with pneumoniae psittaci used as antigens, C. pneumoniae-specific antibodies determined immunoassays. Since presence antimicrobial must be interpreted light their prevalence general population, responses obtained serum samples patients well-defined infection (i.e., positive urethral endocervical DNA amplification) compared to those healthy blood donors. The best sensitivity (86%) specificity 81% was for immunoblotting results, when number individuals > =10 immunoglobulin G (IgG) and/or =2 IgM considered. A 13-kDa antigen recognized most (86% IgG) acute urogenital but rarely (3%) donors (P < 0.0001). results slightly lower than whole which 76 77%, respectively, IgG both hsp60 pgp3