Iron assimilation in plants: reduction of a ferriphytosiderophore by NADH:nitrate reductase from squash

作者: John Smarrelli , Domenic Castignetti

DOI: 10.1007/BF00958970

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摘要: NADH:nitrate reductase (EC 1.6.6.1) from squash (Cucurbita maxima Duch., cv. Buttercup) can catalyze the reduction of a ferriphytosiderophore barley (Hordeum vulgare L. Europa). Maximal activity occurs at pH 6, with an apparentKm andVmax 76 μM and 21 nmol·min-1·(mg protein)-1, respectively. The strongly inhibits nitrate catalyzed by optimal for reduction, i.e. 7.5. On contrary, is poor inhibitor this reaction, 6.0. Thus, has potential to assimilate iron synthesized another plant.

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