Selective Centrilobular Expression of the Aryl Hydrocarbon Receptor in Rat Liver
作者： Magnus Ingelman-Sundberg , Kai O. Lindros , Teija Oinonen , Inger Johansson
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摘要: The aryl hydrocarbon receptor (AHR) is a transcriptional activator of genes encoding group drug-metabolizing enzymes, including cytochrome P450 1A1 ( CYP1A1 ), glutathione S -transferase, tumor-associated aldehyde dehydrogenase and quinone reductase. Both the constitutive inducible expression these in liver zonated, i.e. , dominant hepatocytes centrilobular region, poorly understood position-dependent phenomenon. By comparing cell lysates obtained from opposite acinar regions we observed that immunoreactive AHR protein was almost exclusively confined to cells. mRNA, as analyzed by reverse transcriptase polymerase chain reaction, exhibited similar, although somewhat less pronounced zonation. contrast, only slight zonation nuclear translocator mRNA observed. Treatment rats with omeprazole, an atypical nonligand AHR, caused zone-specific induction region similar seen after pretreatment ligand 3-methylcholanthrene. Our results suggest zone-restricted will allow AHR-regulated but limit their periportal region.
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