Tumoricidal capacities of macrophages photodynamically activated with hematoporphyrin derivative.

摘要: Four days after administration to mice of small amounts (30-600 ng/mouse) hematoporphyrin derivative (HPD), peritoneal macrophages exhibited a greatly enhanced Fc-receptor mediated phagocytic capacity as assayed by ingestion activity IgG-coated sheep erythrocytes. Much higher doses (greater than 3000 did not have this effect. The activated HPD tumoricidal for retinoblastoma cells. We then studied in vitro photodynamic activation white and red fluorescent light irradiation mouse cells (mixture B T lymphocytes) media containing very low concentrations HPD. A short (5 s) exposure (1Wm-2) medium 0.03 ng HPD/mL produced the maximal level macrophages. 15 s 0.1 Thus, with is more efficient that light. This can be explained fact has large absorption peak at about 364 nm which extends into visible range, decreasingly smaller bands 500, 535, 570 630 nm. In photodynamically showed regardless type (white or red) used. These results suggest promotes therapeutic immunopotentiation.