Cross-priming amplification combined with immunochromatographic strip for rapid on-site detection of African swine fever virus
作者： Yao Gao , Xing-Yu Meng , Huawei Zhang , Yuzi Luo , Yuan Sun
DOI: 10.1016/J.SNB.2018.07.164
关键词:
摘要: Abstract African swine fever (ASF) is a highly contagious disease caused by virus (ASFV) in domestic pigs and wild boars. Up to now, no commercial vaccines against ASF are available. With the rapid development of international trade modern logistics frequent activities with Africa, Europe neighboring countries, risk cross-border transmission ASF-free regions increasing. Therefore, there an urgent need establish convenient low-cost diagnostic method for on-site detection timely implement control measures. In this study, cross-priming amplification combination immunochromatographic strip (CPA-strip) was established ASFV. The CPA-strip assay displayed cross-reactivity other viruses. minimum limit 200 copies. Forty-five clinical blood samples collected from Uganda were examined novel assay, 6 out 45 tested positive agreement rate between universal probe library-based real-time PCR 97.8% (44/45). addition, total 100 tissue 57 Chinese herds be negative. We concluded that suitable
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