Potential use of isolated glomeruli and cultured mesangial cells as in vitro models to assess nephrotoxicity.

摘要: The purpose of this short review is to present the potential using isolated glomeruli and cultured mesangial cells as two differentin vitro models assess glomerular effect molecules with nephrotoxic properties. advantage renal that they conserve architecture anatomical region kidney; moreover, are free any vascular, nervous or humoral influences derived from other regions kidney. Mesangial perivascular pericytes located within central portion tuft between capillary loops. have a variety functions including synthesis assembly matrix, endocytosis processing plasma macromolecules, control hemodynamics, mainly ultrafiltration coefficient K f, via cell contraction release vasoactive hormones. Most authors agree play major role in contraction, filtration surface area, f regulation. One effects toxicants on structures contraction. We can quantitatively degree toxicant-induced by measuring changes planar area apparent cross-sectional after exposition toxicant. Thesein also reveal xenobiotics difficult impossible observe vivo. In addition, these studies permit fundamental examination mechanism action cells, possibility at least part their mediated local mediators released cells. mechanisms several such gentamicin, cyclosporin, cisplatin, cadmium As suchin results confirmin vivo hemodynamic caused toxicants, we conclude fruitful tools for study toxicity. systems might serve predictive tool evaluation drugs inducing rate way propose protective agents against dramatic effects.