Competitive organelle-specific adaptors recruit Vps13 to membrane contact sites.
作者： Björn D.M. Bean , Samantha K. Dziurdzik , Kathleen L. Kolehmainen , Claire M.S. Fowler , Waldan K. Kwong
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摘要: The regulated expansion of membrane contact sites, which mediate the nonvesicular exchange lipids between organelles, requires recruitment additional site proteins. Yeast Vps13 dynamically localizes to contacts that connect ER, mitochondria, endosomes, and vacuoles is recruited prospore in meiosis, but its targeting mechanism unclear. In this study, we identify sorting nexin Ypt35 as a novel adaptor recruits endosomal vacuolar membranes. We characterize an interaction motif N terminus related motifs Spo71 mitochondrial protein Mcp1. find Mcp1 for Vps13, Vps13–Mcp1 interaction, not Ypt35, required when ER-mitochondria are lost. All three adaptors compete binding conserved six-repeat region implicated human disease. Our results support competition-based model regulating localization at cellular
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sci-hub.se 参考文章(67)
Yael Elbaz-Alon, Michal Eisenberg-Bord, Vera Shinder, Sebastian Berthold Stiller, Eyal Shimoni, Nils Wiedemann, Tamar Geiger, Maya Schuldiner, Lam6 Regulates the Extent of Contacts between Organelles Cell Reports. ,vol. 12, pp. 7- 14 ,(2015) , 10.1016/J.CELREP.2015.06.022
Luca Rampoldi, Carol Dobson-Stone, Justin P. Rubio, Adrian Danek, Richard M. Chalmers, Nicholas W. Wood, Christine Verellen, Xavier Ferrer, Alessandro Malandrini, Gian M. Fabrizi, Robert Brown, Jeffery Vance, Margaret Pericak-Vance, Gabrielle Rudolf, Sophie Carrè, Elisa Alonso, Michela Manfredi, Andrea H. Németh, Anthony P. Monaco, A conserved sorting-associated protein is mutant in chorea-acanthocytosis Nature Genetics. ,vol. 28, pp. 119- 120 ,(2001) , 10.1038/88821
Charles Elkan, Timothy L. Bailey, Fitting a mixture model by expectation maximization to discover motifs in biopolymers. intelligent systems in molecular biology. ,vol. 2, pp. 28- 36 ,(1994)
Edward L. Huttlin, Lily Ting, Raphael J. Bruckner, Fana Gebreab, Melanie P. Gygi, John Szpyt, Stanley Tam, Gabriela Zarraga, Greg Colby, Kurt Baltier, Rui Dong, Virginia Guarani, Laura Pontano Vaites, Alban Ordureau, Ramin Rad, Brian K. Erickson, Martin Wühr, Joel Chick, Bo Zhai, Deepak Kolippakkam, Julian Mintseris, Robert A. Obar, Tim Harris, Spyros Artavanis-Tsakonas, Mathew E. Sowa, Pietro De Camilli, Joao A. Paulo, J. Wade Harper, Steven P. Gygi, The BioPlex Network: A Systematic Exploration of the Human Interactome Cell. ,vol. 162, pp. 425- 440 ,(2015) , 10.1016/J.CELL.2015.06.043
Sandra Muñoz-Braceras, Rosa Calvo, Ricardo Escalante, TipC and the chorea-acanthocytosis protein VPS13A regulate autophagy in Dictyostelium and human HeLa cells. Autophagy. ,vol. 11, pp. 918- 927 ,(2015) , 10.1080/15548627.2015.1034413
Andrew Murley, Reta D Sarsam, Alexandre Toulmay, Justin Yamada, William A Prinz, Jodi Nunnari, None, Ltc1 is an ER-localized sterol transporter and a component of ER–mitochondria and ER–vacuole contacts Journal of Cell Biology. ,vol. 209, pp. 539- 548 ,(2015) , 10.1083/JCB.201502033
Alberto T Gatta, Louise H Wong, Yves Y Sere, Diana M Calderon-Norena, Shamshad Cockcroft, Anant K Menon, Tim P Levine, None, A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport eLife. ,vol. 4, ,(2015) , 10.7554/ELIFE.07253
Mark S. Longtine, Amos Mckenzie III, Douglas J. Demarini, Nirav G. Shah, Achim Wach, Arndt Brachat, Peter Philippsen, John R. Pringle, Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae Yeast. ,vol. 14, pp. 953- 961 ,(1998) , 10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U
Helen E. Burston, Lymarie Maldonado-Báez, Michael Davey, Benjamen Montpetit, Cayetana Schluter, Beverly Wendland, Elizabeth Conibear, Regulators of yeast endocytosis identified by systematic quantitative analysis Journal of Cell Biology. ,vol. 185, pp. 1097- 1110 ,(2009) , 10.1083/JCB.200811116
Antonio Velayos-Baeza, Andrea Vettori, Richard R. Copley, Carol Dobson-Stone, A.P. Monaco, Analysis of the human VPS13 gene family. Genomics. ,vol. 84, pp. 536- 549 ,(2004) , 10.1016/J.YGENO.2004.04.012