Identification of Multiple Novel Epididymis-Specific β-Defensin Isoforms in Humans and Mice
作者： Yasuhiro Yamaguchi , Takahide Nagase , Ryosuke Makita , Shigetomo Fukuhara , Tetsuji Tomita
DOI: 10.4049/JIMMUNOL.169.5.2516
关键词:
摘要: Defensins comprise a family of cationic antimicrobial peptides that are characterized by the presence six conserved cysteine residues. We identified two novel human β-defensin (hBD) isoforms mining public genomic sequences. The predicted conserve six-cysteine motif identical with hBD-4, termed hBD-5 and hBD-6. also evaluated characteristics mouse homologs hBD-5, hBD-6, HE2β1, (mBD)-12, mBD-11, EP2e (mEP2e). mBD-12 synthetic peptide showed salt-dependent activity. demonstrate epididymis-specific expression pattern mBD-12, mEP2e. In situ hybridization revealed mEP2e in columnar epithelium caput epididymis, contrasting predominant mBD-3 capsule or septum whole epididymis. addition, regional specificity was somewhat overlapping, but not identical, suggesting specific regulation may work for each member family. Our findings indicated multiple specifically cooperatively contribute to innate immunity urogenital system.
highwire.org参考文章(38)
Leona G. Young, Tamara Murphy, Otto Fröhlich, Cecilia Po, Multiple promoter and splicing mRNA variants of the epididymis-specific gene EP2. Journal of Andrology. ,vol. 21, pp. 421- 430 ,(2000) , 10.1002/J.1939-4640.2000.TB03397.X
J. Harder, J. Bartels, E Christophers, J.-M. Schröder, A peptide antibiotic from human skin Nature. ,vol. 387, pp. 861- 861 ,(1997) , 10.1038/43088
Peng Li, Hsiao Chang Chan, Bin He, Siu Cheung So, Yiu Wa Chung, Quan Shang, You-Duan Zhang, Yong-Lian Zhang, An Antimicrobial Peptide Gene Found in the Male Reproductive System of Rats Science. ,vol. 291, pp. 1783- 1785 ,(2001) , 10.1126/SCIENCE.1056545
M Ashburner, C Gissi, B King, G Pesole, J Quackenbush, T Washio, C Bult, D Hill, M Ringwald, C Whittaker, Functional annotation of a full-length mouse cDNA collection Nature. ,vol. 409, pp. 685- 690 ,(2001) , 10.1038/35055500
Clare Fulton, G Mark Anderson, Michael Zasloff, Richard Bull, Anthony G Quinn, Expression of natural peptide antibiotics in human skin The Lancet. ,vol. 350, pp. 1750- 1751 ,(1997) , 10.1016/S0140-6736(05)63574-X
Chin-Chuan Wei, Zhi-Qiang Wang, Qian Wang, Abigail L. Meade, Craig Hemann, Russ Hille, Dennis J. Stuehr, Rapid Kinetic Studies Link Tetrahydrobiopterin Radical Formation to Heme-dioxy Reduction and Arginine Hydroxylation in Inducible Nitric-oxide Synthase Journal of Biological Chemistry. ,vol. 276, pp. 315- 319 ,(2001) , 10.1074/JBC.M008441200
Gillian M Morrison, Donald J Davidson, Julia R Dorin, A novel mouse beta defensin, Defb2, which is upregulated in the airways by lipopolysaccharide FEBS Letters. ,vol. 442, pp. 112- 116 ,(1999) , 10.1016/S0014-5793(98)01630-5
R. Linzmeier, C.H. Ho, B.V. Hoang, T. Ganz, A 450-kb contig of defensin genes on human chromosome 8p23. Gene. ,vol. 233, pp. 205- 211 ,(1999) , 10.1016/S0378-1119(99)00136-5
Yasuhiro Yamaguchi, Shigetomo Fukuhara, Takahide Nagase, Tetsuji Tomita, Shigemi Hitomi, Satoshi Kimura, Hiroki Kurihara, Yasuyoshi Ouchi, A Novel Mouse β-Defensin, mBD-6, Predominantly Expressed in Skeletal Muscle Journal of Biological Chemistry. ,vol. 276, pp. 31510- 31514 ,(2001) , 10.1074/JBC.M104149200
G. Diamond, M. Zasloff, H. Eck, M. Brasseur, W. L. Maloy, C. L. Bevins, Tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian tracheal mucosa: peptide isolation and cloning of a cDNA. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 3952- 3956 ,(1991) , 10.1073/PNAS.88.9.3952