Antigen-driven T cell clones can proliferate in vivo, eradicate disseminated leukemia, and provide specific immunologic memory.

摘要: The aim of the current study was to determine ability antigen-driven cloned helper cell independent cytotoxic T lymphocytes (HITc) proliferate and survive in vivo mediate tumor therapy. HITc clone utilized (denoted 1.B6) specifically cytolytic FBL-3, a syngeneic Friend virus-induced murine leukemia. Activation vitro (48 hr) with FBL-3 induced secretion interleukin 2 (IL 2), expression IL receptors 2R), proliferation. These cells could be "rested" for several weeks without stimulation, which resulted reduced 2R; however, restimulation antigen reinduction 2R examined cyclophosphamide (CY) pretreated donor mice congenic Thy-1 gene. Adoptively transferred found large numbers, were widely distributed 1 wk after transfer. In therapy, 1.B6 when injected into site (i.p.) used as an adjunct CY effective against disseminated FBL-3. this circumstance, recovered from cured 125 days transfer shown lyse response Thus CY, tumor-specific are capable eradicating leukemia, persisting long-term vivo, providing specific immunologic memory.