Taurine ameliorate alloxan induced oxidative stress and intrinsic apoptotic pathway in the hepatic tissue of diabetic rats.

摘要: Oxidative stress is associated with various diabetic complications and taurine plays an important role in ameliorating those difficulties. In the present study we, therefore, investigated whether any beneficial against diabetes induced liver dysfunction if it does, what cellular mechanism follows during protective action. Induction of by alloxan (ALX) (at a dose 120mg/kg body weight, i.p., once) reduced weight plasma insulin level, enhanced blood glucose serum markers related to hepatic injury, accelerated ROS production, disturbed intra-cellular antioxidant machineries disintegrated cells near central vein. This pathophysiology leads apoptotic cell death as evidenced from DNA fragmentation TUNEL aasay. Studies on apoptosis showed that ALX mitochondrial dependent pathway (enhanced cytochrome C release cytosol mitochondria, altered expression Bax, Bcl-2, Apaf-1, caspase-9, caspase-3). Treatment (1% w/v for three weeks) post-hyperglycemia, however, could restore all alteration caused ALX. Moreover, activates PI3Kinase, Akt, hexokinase augments translocation GLUT 2 membrane rats. Combining all, potential therapeutic, may normalize injury.