Reversal effect of Tween-20 on multidrug resistance in tumor cells in vitro

摘要: Multidrug resistance (MDR) is a major barrier for chemotherapy of many cancers. Non-ionic surfactants have great potential to reverse the MDR by preventing onset or delay progression carcinogenic process. However, role Tween-20 in development remains unknown. The aim this study was explore reversal effect and mechanism on tumor cells vitro. Alamar Blue assay used examine index vincristine (VCR), doxorubicin (DOX) 5-fluorouracil (5-FU) KBv200, HepG2/R Bel-7402/5-FU, respectively. Morphological change determined Gimsa Hoechst 33258 staining. acumulation DOX confirmed spectrofluorimetric assay. Cell cycle analysis performed using flow cytometry. mRNA protein expression levels were assessed semiquantitative RT-PCR dot blot, results showed that at concentrations 0.0025%, 0.005%, 0.01% had little cytotoxicity. When combined with cancer drugs, it significantly promoted sensitivity cells. Fluorescence staining percentage apoptotic cell increased when Tween-20. This notion further supported observation treatment potentiated VIN-induced G2/M arrest cycle. Furthermore, intracellular accumulation DOX. blot revealed could downregulate P-glycoprotein. Low can efficiently multidrug phenotype enhancing anticancer drugs. may be via inhibiting multidrug-resistant gene expression.