High-resolution proton magnetic resonance study of porcine colipase and its interactions with taurodeoxycholate.
作者： Tadeusz Wieloch , Bengt Borgstrom , Karl-Erik Falk , Sture Forsen
DOI: 10.1021/BI00575A038
关键词:
摘要: A high-resolution 270-MHz proton NMR study of procine colipase I has been performed, and the resonances in aromatic region spectrum have assigned to amino acid residues by pH titration decoupling experiments. The apparent pKa values three tyrosines were calculated be 10.2, 10.3, 11.8 with one having properties a “buried” residue. tentative assignment primary sequence will discussed. effects taurodeoxycholate (TDC) positively charged deoxycholate derivative on indicate that all histidine are affected bile-salt binding, suggesting TDC molecules bind near these hydrophobic colipase. Measurements calculations line width C (18) methyl group resonance suggest line-width increase this upon interaction large extent can explained as due slower tumbling bound
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