Adhesion of fibronectin, vitronectin, laminin, and collagen type IV to intraocular lens materials in pseudophakic human autopsy eyes. Part 1: histological sections.

摘要: Abstract Purpose To evaluate fibronectin, vitronectin, laminin, and collagen type IV adhesion to poly(methyl methacrylate) (PMMA), silicone, hydrophobic soft acrylate, hydrogel intraocular lenses (IOLs) in pseudophakic human autopsy eyes. Setting Center for Research on Ocular Therapeutics Biodevices, Storm Eye Institute, Medical University of South Carolina, Charleston, USA. Methods Thirty-eight eyes containing PMMA, or IOLs were assessed. Histological sections prepared from each eye, immunohistochemical analyses performed IV. One hundred fifty-two specimens analyzed. Results A sandwich-like structure (anterior posterior capsule/fibronectin/1 cell layer/fibronectin/IOL surface) was seen 12 14 with acrylate IOLs, 3 10 a PMMA IOL ( P = .0094), 1 silicone .0022), 0 4 .0041). The thicker fibrocellular tissue the inner surface anterior capsule that contact lined Vitronectin laminin not found at tissue–IOL interface any specimen. Conclusions This study seems confirm sandwich theory opacification an suggests fibronectin may be major extracellular protein responsible attachment (AcrySof®) capsular bag. represent true bioactive bond between lens epithelial cells bag one reason PCO neodymium:YAG capsulotomy rates are lower IOL.