Simple screening method for molds producing intracellular mycotoxins in pure cultures.

摘要: A simple screening method for molds producing the intracellular mycotoxins brevianamide A, citreoviridin, cyclopiazonic acid, luteoskyrin, penitrem roquefortine C, sterigmatocystin, verruculogen, viomellein, and xanthomegnin was developed. After removing an agar plug from mold culture, mycelium on is wetted with a drop of methanol-chloroform (1:2). By this treatment are extracted within seconds transferred directly to thin-layer chromatography plate by immediately placing while still wet. removal plug, known chromatographic procedures carried out. The substrate (Czapek yeast autolysate agar) growth conditions (25 degrees C 7 days) used Penicillium taxonomists proved suitable production investigated when 60 toxigenic isolates 865 cultures isolated foods feedstuffs were tested method.