Genetic alterations in the S gene of hepatitis B virus in patients with acute hepatitis B, chronic hepatitis B and hepatitis B liver cirrhosis before and after liver transplantation

摘要: : Background: Several studies have shown that hepatitis B immunoglobulin (HBIG) imposes a selection pressure on the virus (HBV) S gene, and emergence of mutations in this region would make reinfection after orthotopic liver transplantation (OLT) possible. Aims: This study was undertaken to analyze presence HBV S-gene different stages infection relationship between HBIG therapy transplant recipients. Methods: The frequency location coding gene were studied by PCR direct sequencing 30 patients (7 with acute self-limited B, 16 chronic 7 recipients for HBV-related end stage disease who became reinfected). Results: average number ammo acid changes higher more advanced disease, 0.57 mutations/100 positions 1.57 (1.28 HBeAg-positive 1.8 anti-HBe-positive patients). substitutions transplanted 2.7 before OLT 3 OLT. No amino detected “a” determinant HBsAg however, 8 observed 6 patients. In patients, 4 samples One these had protective levels anti-HBs, showed additional new OLT, suggesting escape mutant effect therapy. consensus sequences obtained several years transplantation, indicating sequence stability. Conclusion: These results show there is an accumulation end-stage disease. Prophylaxis mainly from highly homogeneous viral population, may be one factor underlying transplantation.