Microsatellite Markers for Classifying and Analysing Genetic Relationship between Banana Cultivars in Indonesia

摘要: In this study, microsatellite markers were used to identify Musa genomic groups and investigate genetic relationships among banana accessions from various areas in Indonesia. One hundred sixteen analysed using MaCIR108 Ma-3-90 primer pairs for identifying groups. Six additional SSR relationship analysis. The results showed that 73 should be classified the AA/AAA AAA groups, two BB group, 21 AAB group 20 ABB group. Ninety-nine out of 116 unique genotypes while rest synonyms. dendrogram generated by UPGMA analysis separated into main clusters with a similarity 0.13. All belonging BB, clustered first cluster, together majority containing pure A genome. second cluster was formed 11 Within B genome according their except four clustering alone. appeared closer than AA could not significantly distinguished, although tended ploidy level. INTRODUCTION Most edible bananas originated wild species, acuminata Colla (2n) balbisiana (2n). Scientifically, cultivars are often referred which categorised on basis levels genomes they contain. Ploidy includes diploids, triploids rare tetraploids, resulting groups: AA, AAA, AAB, ABB, AAAB ... letters representing contribution M. balbisiana, respectively. hybrid evolved natural species mostly triploid (Heslop-Harison Schwarzacher, 2007). Genomic constitution plays an important role classification (Pillay et al., 2004). Cultivar identification based morphological characters is always easy due influence environmental factors. Molecular approaches have been recognised more effective techniques distinguish classify them (Ford-Lloyd 1997). Microsatellites one most informative molecular reveal diversity (Creste 2003, Such genotype many plant (Perera 2001; Chakravarthi Naravaneni, 2006). DNA regions consist short repeat units flanked conserved sequences specific loci (Semagn previous studies banana, several primers shown useful producing diagnostic different (Kaemmer 1997; Creste 2005), determination key application these Proc. Int’l ISHS-ProMusa Symp. Global Perspectives