Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms

摘要: Sampling techniques to detect airborne Salmonella species (spp.) in two pilot scale broiler houses were compared. Broilers inoculated at seven days of age with a marked strain enteritidis. The rearing cycle lasted 42 during the summer. Airborne spp. sampled weekly using impaction, gravitational settling, and impingement techniques. Additionally, on feeders, drinkers, walls, litter. Environmental conditions (temperature, relative humidity, particulate matter (PM) concentration) monitored cycle. presence was determined by culture-dependent molecular methods. No cultivable recovered from poultry houses' surfaces, litter, or air before inoculation. After inoculation, surfaces Were detected impaction settling one weeks after detection based At low concentrations, use for quantification is not recommended. In these cases, combination culture-independent methods These data are valuable improve current measures control transmission pathogens livestock environments optimising sampling practical conditions.