Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions
作者： Haiyan Wang , Zhenzhen Zhang , Xing Xie , Beibei Liu , Yanna Wei
DOI: 10.1128/IAI.00470-20
关键词:
摘要: ABSTRACT Mycoplasma hyopneumoniae is an important respiratory pathogen of pigs that causes persistent and secondary infections. However, the mechanisms by which this occurs are unclear. In study, we established air-liquid interface culture systems for pig bronchial epithelial cells (ALI-PBECs) were comparable to conditions in native bronchus vivo. We used ALI-PBECs model study infection migration characteristics M. hyopneumoniaein vitro. Based on results, confirmed was able adhere disrupt mucociliary function. Importantly, could migrate basolateral chamber through paracellular route but not transcellular pathway, achieved reversibly disrupting tight junctions (TJs) increasing permeability damaging integrity barrier. examined ability using first time. The disruption barrier allowed route, may be related immune evasion, extrapulmonary dissemination, hyopneumoniae.
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