Prevalence of rotavirus on high-risk fomites in day-care facilities.

摘要: Study objective. The objective of this study was to determine the prevalence rotavirus contamination on environmental surfaces in day-care environments, using polymerase chain reaction technique. Design. High-risk fomites were identified two centers and sampled biweekly during a 6-month period. Water samples from water-play tables each center also collected During an infectious disease outbreak, rooms which outbreak occurred. Reverse transcriptase/polymerase carried out for viral detection fomites, standard bacteriologic measures used detect bacteria tables. Results. A total 96 fomite tested presence centers, 18/96 (19%) positive rotavirus. timing differed between centers. In that housed infants, peak rotavirus-positive coincided with enteric outbreaks. Rotavirus found telephone receiver, drinking fountain, table, toilet handles both Bacteria large quantities table samples. Conclusions. Moist including telephone, water fountains, are common sources within environment. Until safe affordable drug or vaccine against is available general use, avoidance rotaviral infections most effective method prevention gastroenteritis.