MiR-29a promotes intestinal epithelial apoptosis in ulcerative colitis by down-regulating Mcl-1.
作者： Zhengkun Hou , Fengbin Liu , Bo Lv , Zhihui Liu , Jiangtao Hou
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摘要: Objective While it's widely accepted that the etiology of ulcerative colitis (UC) involves both genetic and environmental factors, pathogenesis is still poorly understood. Intestinal epithelial apoptosis one most common histopathological changes UC expression a number genes may contribute to progression UC. MicroRNAs have recently emerged as powerful regulators diverse cellular processes been shown be involved in many immune-mediated disorders such psoriasis, rheumatoid arthritis, lupus, asthma. A unique microRNA profile has identified UC, suggesting that, microRNAs play an important role We investigated miR-29a intestinal Methods The Mcl-1, anti-apoptotic BCL-2 family member, was evaluated patients mice model induced by dextran sodium sulfate (DSS). rate cells also evaluated. Results In DSS-induced mice, were up-regulated down-regulated, respectively. binding site (7 nucleotides) on 3'UTR mcl-1 mutation this led mis-match between mcl-1. Knockout Mcl-1 caused colonic HT29 cells. addition, regulated down-regulating Mcl-1. Conclusion regulating via
参考文章(40)
K M Das, L H Griffel, Ulcerative colitis: pathogenesis, diagnosis, and current treatment. Journal of the Association for Academic Minority Physicians : the official publication of the Association for Academic Minority Physicians. ,vol. 7, pp. 63- 69 ,(1996)
J. E. Reynolds, A. Eastman, R. W. Craig, J. D. Jenkinson, Liping Qian, Ping Zhou, Tao Yang, Mcl-1, a Member of the Bcl-2 Family, Delays Apoptosis Induced by c-Myc Overexpression in Chinese Hamster Ovary Cells Cancer Research. ,vol. 54, pp. 6348- 6352 ,(1994)
David Bernardo, Sara Vallejo-Díez, Elizabeth R. Mann, Hafid O. Al-Hassi, Beatriz Martínez-Abad, Enrique Montalvillo, Cheng T. Tee, Aravinth U. Murugananthan, Henar Núñez, Simon T. C. Peake, Ailsa L. Hart, Luis Fernández-Salazar, Jose A. Garrote, Eduardo Arranz, Stella C. Knight, IL-6 promotes immune responses in human ulcerative colitis and induces a skin-homing phenotype in the dendritic cells and Tcells they stimulate. European Journal of Immunology. ,vol. 42, pp. 1337- 1353 ,(2012) , 10.1002/EJI.201142327
John H. Kwon, Sushila R. Dalal, The Role of MicroRNA in Inflammatory Bowel Disease. Gastroenterología y Hepatología. ,vol. 6, pp. 714- 722 ,(2010)
Kazuko Shichijo, Mutsumi Matuu, Yuji Ikeda, Shinji Naito, Masahiro Ito, Ichiro Sekine, Clinicopathologic study of dextran sulfate sodium experimental murine colitis Laboratory Investigation. ,vol. 69, pp. 238- 249 ,(1998) , 10.1016/S0021-5198(19)41298-5
J L Mott, S Kobayashi, S F Bronk, G J Gores, mir-29 regulates Mcl-1 protein expression and apoptosis Oncogene. ,vol. 26, pp. 6133- 6140 ,(2007) , 10.1038/SJ.ONC.1210436
Witold Filipowicz, Suvendra N. Bhattacharyya, Nahum Sonenberg, Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nature Reviews Genetics. ,vol. 9, pp. 102- 114 ,(2008) , 10.1038/NRG2290
Rhonda M. Perciavalle, Joseph T. Opferman, Delving deeper: MCL-1's contributions to normal and cancer biology Trends in Cell Biology. ,vol. 23, pp. 22- 29 ,(2013) , 10.1016/J.TCB.2012.08.011
Yang Wang, Xinwei Zhang, Hui Li, Jinpu Yu, Xiubao Ren, The role of miRNA-29 family in cancer. European Journal of Cell Biology. ,vol. 92, pp. 123- 128 ,(2013) , 10.1016/J.EJCB.2012.11.004
Georgios Koukos, Christos Polytarchou, Jess L. Kaplan, Alessio Morley–Fletcher, Beatriz Gras–Miralles, Efi Kokkotou, Mariah Baril–Dore, Charalabos Pothoulakis, Harland S. Winter, Dimitrios Iliopoulos, MicroRNA-124 Regulates STAT3 Expression and Is Down-regulated in Colon Tissues of Pediatric Patients With Ulcerative Colitis Gastroenterology. ,vol. 145, pp. 842- 852 ,(2013) , 10.1053/J.GASTRO.2013.07.001