Markers of Pulmonary Oxygen Toxicity in Hyperbaric Oxygen Therapy Using Exhaled Breath Analysis.

摘要: Introduction Although hyperbaric oxygen therapy (HBOT) has beneficial effects, some patients experience fatigue and pulmonary complaints after several sessions. The current limits of exposure to prevent toxicity (POT) are based on function tests (PFT), but the limitations PFT recognized worldwide. However, no newer modalities detect POT have been established. Exhaled breath analysis in divers shown volatile organic compounds (VOCs) inflammation methyl alkanes. This study hypothesized that similar VOCs might be detected HBOT. Methods Ten healthy volunteers Royal Netherlands Navy underwent six HBOT sessions (95 min at 253 kPa, including three 5-min "air breaks"), i.e., five consecutive days followed by another session 2 rest. At 30 before dive, min, 4 h post-dive, exhaled was collected PFT. samples were analyzed using gas chromatography-mass spectrometry (GC-MS). After univariate correlation retention times, ion fragments could identified a reference database. Using these reconstructed, which clustered principal component analysis. These clusters tested longitudinally with ANOVA. Results GC-MS analysis, eleven relevant into two components (PC). PC1 consisted associated showed significant change over time. intensities PC2, consisting alkanes, decrease (p = 0.001) first 50.8%, remained decreased during subsequent (mean 82%), even further rest 58% (compared baseline). virtually unchanged. Discussion found when compared diving, alkanes (PC2) is contrast increase seen divers. It unknown why emission (which originate from phosphatidylcholine membrane alveoli) reduced suggests not as damaging tract previously assumed. Future research should focus (inflammation alkanes).