Confocal microscopy as a tool for studying protein adsorption to chromatographic matrices
作者： Anders Ljunglöf , Rolf Hjorth
DOI: 10.1016/0021-9673(96)00290-7
关键词:
摘要: Confocal scanning laser microscopy was used for studying protein adsorption to affinity chromatography matrices. The of Protein A IgG Sepharose 6 Fast Flow studied by batch incubation with varying amounts fluorescently labeled A. At low sample amounts, had been adsorbed a thin outer layer. By increasing the sample-IgG ratio, layer also increased. Likewise, depth dependent on time. Finally, stack confocal images separated in space three-dimensional reconstruction pattern particle.
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